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A COMPARISON OF TWO INCUBATION TEMPERATURES FOR THE ISOLATION OF GRAM‐NEGATIVE CONTAMINANTS FROM RAW MATERIALS AND NON‐STERILE PHARMACEUTICALS
Author(s) -
Ferguson Anne,
Patel Asmita,
Baird Rosamund M.
Publication year - 1987
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1987.tb00534.x
Subject(s) - contamination , incubation , gram , raw material , isolation (microbiology) , recovery rate , chemistry , incubation period , bacteria , toxicology , chromatography , food science , environmental chemistry , microbiology and biotechnology , biology , organic chemistry , ecology , biochemistry , genetics
SUMMARY Selective and non‐selective broth enrichment techniques may be used in the isolation of microbial contaminants from pharmaceutical products. A non‐selective method may give better recovery rates for damaged organisms. A trial was carried out to determine whether the recovery of Gram‐negative contaminants could be improved by using an incubation temperature of 30°C for 48 h, rather than the more widely used 37°C for 24 h. Contaminants were isolated from 3.2% of samples incubated at the lower temperature compared with 0.8% at the higher temperature. The recovery rate from raw materials improved noticeably (9.0% compared with 0.9%).