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EFFECT OF STERILIZATION BY AUTOCLAVING ON THE STABILITY OF EYE DROPS OF PILOCARPINE HYDROCHLORIDE AND PHYSOSTIGMINE SULPHATE
Author(s) -
Mair Ann E.,
Miller J. H. McB.
Publication year - 1984
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1984.tb01079.x
Subject(s) - pilocarpine , physostigmine , chromatography , sterilization (economics) , chemistry , hydrochloride , pharmacology , medicine , organic chemistry , acetylcholine , psychiatry , monetary economics , economics , epilepsy , foreign exchange market , foreign exchange
SUMMARY A high‐performance liquid chromatographic assay is described for the simultaneous assay of pilocarpine and physostigmine in the presence of their decomposition products. This assay method was used to quantitatively determine these medicaments in an eye drop formulation which had been sterilized by autoclaving at 115° for 30 min and at 121° for 15 min. There was no significant loss of pilocarpine whereas physostigmine was degraded. The major decomposition product is shown to be eseroline.