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HIGH PRESSURE LIQUID CHROMATOGRAPHIC STABILITY STUDY OF LACHESINE CHLORIDE IN EYE DROPS
Author(s) -
Collins A. J.,
Burbridge T. A.,
Bain R.
Publication year - 1980
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1980.tb00966.x
Subject(s) - chromatography , chemistry , high performance liquid chromatography , sodium , methanol , chloride , heptane , drop (telecommunication) , sterilization (economics) , eye drop , organic chemistry , computer science , monetary economics , economics , foreign exchange market , ophthalmology , foreign exchange , medicine , telecommunications
A specific HPLC assay method for lachesine chloride has been developed and used to study the stability of lachesine in eye drop formulations. The samples were run on a C8 column with methanol 60% v/v, water 40% v/v containing 0.005 M heptane sulphonic acid sodium salt as an ion‐pairing agent at pH 3.5. Using infra‐red spectroscopy the major breakdown product on heat sterilization of the eye drop formulations was found to be benzillic acid.