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A GAS‐CHROMATOGRAPHIC ASSAY OF CHLORAMPHENICOL APPLICATION TO FORMULATION AND TO SAMPLES SHOWING HYDROLYTIC OR PHOTOCHEMICAL DEGRADATION
Author(s) -
Irwin W. J.,
Po A. Li Wan,
Wadhwani R. R.
Publication year - 1980
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1980.tb00950.x
Subject(s) - chloramphenicol , derivatization , chemistry , degradation (telecommunications) , hydrolysis , chromatography , decomposition , gas chromatography , kinetics , chemical decomposition , high performance liquid chromatography , organic chemistry , biochemistry , antibiotics , telecommunications , computer science , physics , quantum mechanics
A gas‐chromatographic method for the rapid assay of chloramphenicol in pharmaceutical preparations is described. The procedure involves the preparation of a cyclic phenylboronate ester and the derivatization and analysis stages each occupy only 5 min. The method is applicable to the determination of chloramphenicol in solid, semi‐solid and liquid dosage forms. Degraded samples, produced either through hydrolysis or photochemical decomposition may also be monitored and the kinetics of the degradation may be followed. This has been exemplified by the determination of the degree of protection from photochemical degradation offered to chloramphenicol eye‐drops by various containers. The photochemical decomposition was also followed by high‐performance liquid chromatography.