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HIGH PRESSURE LIQUID CHROMATOGRAPHIC DETERMINATION OF CHLORHEXIDINE ACETATE AND SOME TROPANE ALKALOIDS IN EYE DROPS
Author(s) -
Collins A. J.,
Campbell A. T.,
Bain R.
Publication year - 1979
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1979.tb00157.x
Subject(s) - chemistry , chromatography , methanol , chlorhexidine , tropane , solvent , heptane , ethyl acetate , salt (chemistry) , organic chemistry , medicine , dentistry
Summary A method for the determination of chlorhexidine acetate in eye drops has been developed. Samples are assayed by direct injection on a C8 reverse phase column. The mobile phase is methanol 80% v/v, water 20% v/v with 0005M heptane sulphonic acid sodium salt at pH 3–5 as an ion‐pairing agent. When run on this system the chlorhexidine is well separated from/7‐chloroaniline, its major breakdown product. This method also allows the alkaloids to be determined by direct injection using approximately methanol 50% v/v, water 50% v/v with the ion‐pairing agent at pH 3–5. The procedure is to first assay the alkaloids and then by adjusting the solvent to methanol 80% v/v water 20% v/v and re‐injecting the samples, the chlorhexidine in the eye drops is determined. The samples are detected using a variable wavelength spectrophotometer.