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CONTAMINATING SUBSTANCES IN UROKINASE PREPARATIONS
Author(s) -
Nobuhara Masahiro,
Ashida Yoshikazu,
Ohnishi Haruo
Publication year - 1979
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1979.tb00154.x
Subject(s) - lysozyme , biological activity , urokinase , chemistry , coagulation , chromatography , size exclusion chromatography , antigen , biochemistry , immunology , biology , enzyme , medicine , in vitro , surgery
Summary Urokinase (UK) preparations, obtained at random on the commercial market from ten manufacturers, were investigated for their degree of purification by measurement of their coagulant activity, lysozyme activity, kinin‐ and brady‐kinin‐like activity, endotoxin content and hepatitis B surface antigen (HBs antigen). The results indicated that the UK preparations could be divided into two groups: ‘clean’ UK which contained no detectable biologically active substances, and ‘dirty’ UK which contained abundant biological active substances. When ‘dirty’ UK containing high procoagulant activity was injected into rabbits, the coagulation system was activated. Separation of the pro‐coagulant activity from the UK components may be difficult on an industrial scale by gel filtration. Since ‘clean’ UK contains no detectable biologically active substances, its specific activity was the highest among the UK preparations.