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High pressure liquid chromatographic determination of phenylmercuric nitrate in eye‐drops
Author(s) -
Mehta A. C.,
Midcalf B.,
Hetherington C.
Publication year - 1976
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1976.tb00063.x
Subject(s) - chromatography , chemistry , nitrate , eye drop , ophthalmology , medicine , organic chemistry
Summary A rapid and sensitive method for the determination of phenylmercuric nitrate has been developed. After extraction with chloroform, the sample is evaporated to dryness and redissolved in the small amount of chloroform. Injection is made on a silica gel column at 4130 kPa. The mobile phase is hexane‐ethanol (9:1) and the phenylmercuric nitrate is detected at 230 nm. Peak areas relative to an internal standard (chloroform) are used to calculate the unknown concentration. The detection limit is about 1–5 times 10 ‐3 g/l and the samples are analysed with errors of less than 10%. The proposed method is applicable to solutions of phenylmercuric nitrate and certain eye‐drops.