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Preparation of a stannous methylene diphosphonate kit and its biological distribution in animals
Author(s) -
Khan Ralph Ashraf Andrew,
Sheasby Barbara
Publication year - 1976
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1976.tb00061.x
Subject(s) - reagent , methylene , labelling , chemistry , chromatography , thin layer chromatography , chelation , yield (engineering) , reproducibility , distribution (mathematics) , active compound , radiochemistry , nuclear chemistry , organic chemistry , materials science , biochemistry , mathematical analysis , mathematics , metallurgy
Summary A method is described for the preparation of a freeze‐dried stannous methylene diphosphonate kit* (Sn‐MDP) suitable for use in bone scanning after complexing with 99m Tc. Several combinations of the reagents were used, and these resulted in different levels of bone and soft tissue localization. Two of such combinations produced a kit which localized predominantly in bone, and of these only one showed consistent batch to batch reproducibility. The biological distribution of the labelled compound has been tested in animals, and the results obtained at 2 h post‐injection are presented. The shelf‐life has been found to be in excess of 3 months, and quality control using thin layer chromatography has shown a labelling yield >95%.