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A contribution to the application of the Limulus test for demonstrating the absence of pyrogenicity in saline and dextrose solutions prepared in hospitals
Author(s) -
Izgü Enver,
Çelik Süleyman
Publication year - 1976
Publication title -
journal of clinical pharmacy and therapeutics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.622
H-Index - 73
eISSN - 1365-2710
pISSN - 0269-4727
DOI - 10.1111/j.1365-2710.1976.tb00056.x
Subject(s) - saline , limulus , distilled water , medicine , sterile water , incubation , anesthesia , chromatography , chemistry , biology , biochemistry , food science , paleontology
Summary The Limulus test for pyrogens was performed on the saline and dextrose solutions prepared in the parenteral products laboratory of the hospital and these solutions were administered to 100 patients under standardized conditions and to 500 patients under clinical conditions. Success in using the Limulus test depends on undisturbed incubation, good manufacturing practice and a positive endotoxin control system. It is useful to employ a positive control with stale or stored distilled water and with tap water, together with endotoxin. The results showed that the Limulus test for pyrogens can be considered as a sensitive and convenient test for the absence of pyrogenicity in saline and dextrose solutions prepared in a well organized laboratory of a hospital.