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Anti‐ C ampylobacter and resistance‐modifying activity of A lpinia katsumadai seed extracts
Author(s) -
Klančnik A.,
Gröblacher B.,
Kovač J.,
Bucar F.,
Možina S. Smole
Publication year - 2012
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2012.05424.x
Subject(s) - alpinia , efflux , biology , campylobacter , microbiology and biotechnology , traditional medicine , botany , bacteria , biochemistry , medicine , genetics
Aims We tested extracts from A lpinia katsumadai seeds for anti‐ C ampylobacter activity and investigated the roles of the C me ABC and C me DEF efflux pumps in C ampylobacter resistance to these natural phenolics. Additionally, we investigated an A . katsumadai ethanolic extract (AlpE) and other plant extracts as putative efflux pump inhibitors on C ampylobacter isolates and mutants in efflux pump genes. Methods and Results A lp E showed antimicrobial activity against sensitive and multidrug‐resistant C ampylobacter isolates. CmeB inactivation resulted in the greatest reduction in resistance, while cmeF and cmeR mutations produced only moderate effects on minimal inhibitory concentrations ( MIC s). The chemical efflux pump inhibitors additionally reduced MIC s in isolates and mutants, confirming that active efflux is an important mechanism in resistance to AlpE, with additional contributions of other efflux systems. A notable decrease in resistance to tested antimicrobials in the presence of subinhibitory concentrations of AlpE confirms its modifying activity in C ampylobacter spp. Conclusions A lp E is important anti‐ C ampylobacter source of antimicrobial compounds with resistance‐modifying activity. At least two of the efflux systems are involved in the resistance to A . katsumadai antimicrobial seed extracts. Significance and Impact of the Study This is the first report of antimicrobial and resistance‐modifying activity of A lp E from A . katsumadai seeds, demonstrating its potential in the control of C ampylobacter in the food chain.