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Characterizing a novel strain of B acillus amyloliquefaciens BAC 03 for potential biological control application
Author(s) -
Meng Q.X.,
Jiang H.H.,
Hanson L.E.,
Hao J.J.
Publication year - 2012
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2012.05420.x
Subject(s) - bacillus amyloliquefaciens , common scab , antimicrobial , ammonium sulfate precipitation , strain (injury) , bacillus (shape) , streptomyces , biology , microbiology and biotechnology , food science , biological pest control , bacteria , agar , horticulture , fermentation , biochemistry , genetics , anatomy , enzyme , size exclusion chromatography
Aims To identify and characterize a bacterial strain BAC 03, evaluate its biological control activity against potato common scab ( S treptomyces spp.) and characterize an antimicrobial substance produced by BAC 03. Methods and Results Bacterial strain BAC 03, isolated from potato common scab suppressive soil, was identified as B acillus amyloliquefaciens by analysing sequences of fragments of the rec A , rec N , che A and gyr A genes. BAC 03 displayed an antagonistic activity against S treptomyces spp. on agar plates using a co‐culture method. In glasshouse assays, BAC 03 applied in potting mix significantly reduced common scab severity ( P < 0·05) and potentially increased the growth of potato plants ( P < 0·05). An antimicrobial substance extracted from BAC 03 by ammonium sulfate precipitation was identified as an LCI protein using liquid chromatography–mass spectrometry. The antimicrobial activity of either a BAC 03 liquid culture or the ammonium sulfate precipitate fraction was stable under a wide range of temperatures, and pH levels, as well as following incubation with several chemicals, but was reduced by all proteinases tested. Conclusions Bacillus amyloliquefaciens strain BAC 03 displayed a strong antimicrobial activity, that is, the suppression of potato common scab, and may potentially enhance the plant growth. LCI protein is associated with some of the antimicrobial activity. Significance and Impact of the Study Bacterial strain BAC 03 has the potential to be developed as a commercial biological control agent for potato common scab.