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Plant growth promoting potential and soil enzyme production of the most abundant S treptomyces spp. from wheat rhizosphere
Author(s) -
Jog R.,
Nareshkumar G.,
Rajkumar S.
Publication year - 2012
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2012.05417.x
Subject(s) - rhizosphere , biology , chitinase , cellulase , streptomyces , incubation , enzyme assay , yeast extract , fermentation , botany , food science , enzyme , bacteria , biochemistry , genetics
Aim To evaluate the plant growth promotion ( PGP ) potential and soil enzyme production under solid state fermentation ( SSF ) by most abundant S treptomyces spp. isolated from the wheat rhizosphere and to evaluate their effect on plant growth parameters. Methods and Results Actinomycetes were isolated from wheat rhizosphere and screened for PGP activities. Three actinomycete isolates having significantly higher PGP activities ( S treptomyces rochei IDWR 19, S treptomyces carpinensis IDWR 53, S treptomyces thermolilacinus IDWR 81) were selected. The soil enzymes production potential of these isolates using soil extract and wheat straw under ssf was assessed. Utilization of soil extract as a fermentation medium for soil enzyme production by A ctinomycetes has been reported first time in this study. Maximum chitinase ( S . rochei IDWR 19 12·2 U mg −1 protein) and phytase activity ( S . carpinensis IDWR 53 5·2 U mg −1 protein) was produced on 7th day of incubation, whereas maximum alkaline protease ( S . rochei IDWR 19 3·2 U mg −1 protein) was produced on 6th day of incubation. For cellulase ( S. rochei IDWR 19 7·4 U mg −1 protein) and invertase ( S . carpinensis IDWR 53 451 U mg −1 protein) maximum activity was observed on 4th as well as 5th day of incubation. On the basis of PGP activity and enzyme production, two actinomycete isolates ( S . rochei IDWR 19 and S . thermolilacinus IDRWR 81) were selected for plant growth experiment. An increase of 12·2 and 24·5% in shoot length of plants inoculated with S. rochei IDWR 19 and S . thermolilacinus IDWR 81 was observed, respectively. A similar increase in biomass of 1·8‐ and 2·3‐fold was also recorded for the two isolates, respectively. Conclusions It could be concluded that S treptomyces sp. with high PGP activities and soil enzyme production capability significantly improved growth and development of wheat cv. Significance and Impact of the Study The abundant A ctinomycetes obtained in this study ( S . rochei IDWR 19 and S . thermolilacinus IDWR 81) are rhizosphere competent and effective strains.