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A new real‐time PCR method for the identification of S almonella Dublin
Author(s) -
Persson S.,
Jacobsen T.,
Olsen J.E.,
Olsen K.E.P.,
Hansen F.
Publication year - 2012
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2012.05378.x
Subject(s) - library science , clinical microbiology , danish , medicine , political science , biology , computer science , microbiology and biotechnology , philosophy , linguistics
Aims Development of a real‐time PCR method for the specific detection of S almonella Dublin. Methods and Results The method was directed towards a S alm . Dublin‐specific sequence of the vagC gene on the S almonella virulence plasmid (p SDV ) and towards S almonella genus‐specific sequence of the invA gene, serving as an internal amplification control. The method showed 100% inclusivity and exclusivity when tested on a strain collection containing 50 serotyped S . Dublin strains, 20 strains of other S almonella serotypes and 10 non‐ S almonella strains. The method also showed 100% inclusivity and 99% exclusivity in a collaborative study comprising eight laboratories, where each laboratory received ten different S . Dublin strains and 10 other S almonella serotypes. Conclusions The method showed excellent performance both when validated in the laboratory and in the collaborative study. Significance and Impact of the Study Application of the present method in food control, for example at slaughterhouses, can improve the contamination control of this veterinary and clinically important S almonella serotype.