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Variability in the concentrations of volatile metabolites emitted by genotypically different strains of P seudomonas aeruginosa
Author(s) -
Shestivska V.,
Španěl P.,
Dryahina K.,
Sovová K.,
Smith D.,
Musílek M.,
Nemec A.
Publication year - 2012
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2012.05370.x
Subject(s) - pseudomonas aeruginosa , chemistry , mass spectrometry , acetone , chromatography , isoprene , volatile organic compound , strain (injury) , butanone , gas chromatography–mass spectrometry , microbiology and biotechnology , pseudomonadaceae , exhalation , breath gas analysis , food science , environmental chemistry , bacteria , biology , biochemistry , organic chemistry , genetics , anatomy , solvent , copolymer , polymer
Aims To characterize the volatile metabolites produced by genotypically diverse strains of P seudomonas aeruginosa in order to evaluate their potential for use as biomarkers of lung infection in noninvasive breath analysis. Methods and Results Volatile organic compounds ( VOC s) emitted from 36 clinical strains of P s. aeruginosa (belonging to different multilocus sequence types) cultured in liquid and on solid media were analysed by gas chromatography mass spectrometry ( GC ‐ MS ) and selected ion flow tube mass spectrometry ( SIFT ‐ MS ). Several previously identified VOC s were detected, including ethanol, acetone, 2‐butanone, 2‐pentanone, isoprene, aminoacetophenone, dimethyl sulphide, dimethyl disulphide, dimethyl trisulphide and methyl thiocyanate. Additionally, significant production of 3‐methyl‐butanone, acetophenone, methylthioacetate and methyl thiobutanoate was observed for the first time in this study. SIFT ‐ MS quantifications of VOC s showed high variability between genotypically distinct strains. Conclusions The data obtained indicate that the production rates of the volatile biomarkers of P s. aeruginosa vary by two orders of magnitude between different strains cultured under the same conditions. Similar variability was observed for both liquid and solid media. Significance and Impact of the Study Inter‐strain genotypic variability strongly influences the concentrations of the volatile biomarkers from P s. aeruginosa . A group of several biomarkers quantified in real time in exhaled breath may thus provide a more valuable indicator of the course of pulmonary infections compared to a single biomarker.