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Biodiesel synthesis by a one‐step method in a genetically engineered E scherichia coli using rice straw hydrolysate and restaurant oil wastes as raw materials
Author(s) -
Wang B.,
Lin H.,
Zhan J.,
Yang Y.,
Zhou Q.,
Zhao Y.
Publication year - 2012
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2012.05357.x
Subject(s) - hydrolysate , biodiesel , raw material , escherichia coli , straw , rice straw , microbiology and biotechnology , bioenergy , biofuel , genetically engineered , food science , pulp and paper industry , genetically modified organism , waste management , environmental science , chemistry , biology , agronomy , engineering , biochemistry , hydrolysis , organic chemistry , gene , catalysis
Aims To reduce the cost of biodiesel, the utilization of rice straw hydrolysate and restaurant oil wastes as the substrates for biodiesel production in a recombinant strain by a one‐step method was investigated. Methods and Results A recombinant E scherichia coli p ET 28a (+)‐ PAW , which encodes enzymes for the ethanol pathway and acyltransferase, was constructed. Fermentation results in L uria‐ B ertani medium revealed that xylose was favourable for fatty acid ethyl esters ( FAEE s) production (2·79 g l −1 ) by E . coli p ET 28a (+)‐ PAW . When rice straw hydrolysate and restaurant oil wastes were utilized as the monosaccharide substituent and the fatty acids substituent, 0·27 g l −1 FAEE s and 0·249 g l −1 FAEE s were obtained, respectively. 1·27 g l −1 FAEE s was obtained in the restaurant oil wastes medium supplemented by 2% sodium oleate. Conclusions The fermentation results indicated that the strain was effective in biodiesel production. The rice straw hydrolysate and restaurant oil wastes could be utilized by E . coli as the substrates for FAEE production. Significance and Impact of the Study This novel exploration might pave the way for biodiesel production from waste materials by genetically engineered microorganism.