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Pseudomonas M162 confers protection against rainbow trout fry syndrome by stimulating immunity
Author(s) -
Korkeaaho T.L.,
Papadopoulou A.,
Heikkinen J.,
von Wright A.,
Adams A.,
Austin B.,
Thompson K.D.
Publication year - 2012
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2012.05325.x
Subject(s) - stirling engine , wright , library science , aquaculture , medicine , fish <actinopterygii> , history , art history , engineering , biology , computer science , fishery , mechanical engineering
Aims: To evaluate the antagonistic effect of Pseudomonas M162 against Flavobacterium psychrophilum . Methods and Results: The antagonistic activity of M162 was tested in vivo and in vitro , and its mode of action examined by siderophore production and immunological responses of rainbow trout ( Oncorhynchus mykiss ) fry. Pseudomonas M162 inhibited the growth of Fl. psychrophilum in vitro and increased the resistance of the fish against the pathogen, resulting in a relative per cent survival (RPS) of 39·2%. However, the siderophores produced by M162 did not have an inhibitory effect on Fl. psychrophilum . In fish fed with M162, the probiotic colonized the gastrointestinal tract and stimulated peripheral blood leucocyte counts, serum lysozyme activity and total serum immunoglobulin levels after 3 weeks from the start of feeding. Conclusions: This study showed the potential of Pseudomonas M162 as a probiotic by reducing the mortalities that occurred during an experimental Fl. psychrophilum infection, resulting mainly through the immunostimulatory effects of the bacterium. Significance and Impact of the Study: Rainbow trout fry syndrome (RTFS) causes high mortalities during the early life stages of the fish’s life cycle, partly because their adaptive immunity has not yet fully developed. Thus, immunomodulation by probiotics could be an effective prophylactic method against RTFS.