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Outer membrane defect and stronger biofilm formation caused by inactivation of a gene encoding for heptosyltransferase I in Cronobacter sakazakii ATCC BAA‐894
Author(s) -
Wang L.,
Hu X.,
Tao G.,
Wang X.
Publication year - 2012
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2012.05263.x
Subject(s) - christian ministry , china , library science , food microbiology , political science , biology , computer science , law , genetics , bacteria
Aims: To investigate the role of lipopolysaccharide (LPS) structure in the stability of outer membrane and the ability of biofilm formation in Cronobacter sakazakii. Methods and Results: A C. sakazakii mutant strain LWW02 was constructed by inactivating the gene ESA_04107 encoding for heptosyltransferase I. LPS were purified from LWW02, and changes in their structure were confirmed by thin‐layer chromatography and electrospray ionization mass spectrometry. Comparing with the wild‐type strain BAA‐894, slower growth, higher membrane permeability, higher surface hydrophobicity, stronger ability of autoaggregation and biofilm formation were observed for the mutant strain LWW02. Conclusions: The gene ESA_04107 encodes heptosyltransferase I in C. sakazakii ATCC BAA‐894. The cleavage of LPS in C. sakazakii could cause its outer membrane defects and increase its ability to form biofilms. Significance and Impact of the Study: The study is important for understanding the pathogenic mechanism and efficient control of C. sakazakii .