Research Library

Premium Differentiation of live, dead and treated cells of Escherichia coli O157:H7 using FT‐IR spectroscopy
Davis R.,
Deering A.,
Burgula Y.,
Mauer L.J.,
Reuhs B.L.
Publication year2012
Publication title
journal of applied microbiology
Resource typeJournals
PublisherBlackwell Publishing Ltd
Abstract Aims:  To apply specific collection techniques and spectroscopy to differentiate between live and dead Escherichia coli O157:H7 cells, as well as cells subjected to various inactivation treatments, including heat, salt, UV, antibiotics and alcohol. Methods and Results:  Fourier transform‐infrared (FT‐IR) spectroscopy was used to analyse E. coli O157:H7 cells, after filtration or immunomagnetic collection. Partial least squares analysis of the spectra quantified live E. coli O157:H7 in the presence of dead cells with an R 2  > 0·996. Canonical variate analysis (CVA) not only differentiated between spectra of 100% dead and 100% live cells but also between 1% live : 99% dead and 100% dead. CVA using principal components also differentiated between the spectra of the differentially treated cells at a 95% confidence level, and Cooman plots showed clear separation between clusters of spectra of bacteria exposed to the different inactivation treatments. Mahalanobis distances (MD) corroborated the results of CVA. Conclusions:  These results demonstrated the effectiveness of rapid cell collection and FT‐IR spectroscopy techniques to differentiate between live and dead E. coli O157:H7 cells. Significance and Impact of the Study:  This technique has potential applications for use with foods subjected to various inactivation treatments.
Subject(s)bacteria , biochemistry , biology , chemistry , enterobacteriaceae , escherichia coli , gene , genetics , microbiology and biotechnology , physics , quantum mechanics , spectroscopy
SCImago Journal Rank0.889

Seeing content that should not be on Zendy? Contact us.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here