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Premium Differentiation of live, dead and treated cells of Escherichia coli O157:H7 using FT‐IR spectroscopy
Author(s)
Davis R.,
Deering A.,
Burgula Y.,
Mauer L.J.,
Reuhs B.L.
Publication year2012
Publication title
journal of applied microbiology
Resource typeJournals
PublisherBlackwell Publishing Ltd
Abstract Aims:  To apply specific collection techniques and spectroscopy to differentiate between live and dead Escherichia coli O157:H7 cells, as well as cells subjected to various inactivation treatments, including heat, salt, UV, antibiotics and alcohol. Methods and Results:  Fourier transform‐infrared (FT‐IR) spectroscopy was used to analyse E. coli O157:H7 cells, after filtration or immunomagnetic collection. Partial least squares analysis of the spectra quantified live E. coli O157:H7 in the presence of dead cells with an R 2  > 0·996. Canonical variate analysis (CVA) not only differentiated between spectra of 100% dead and 100% live cells but also between 1% live : 99% dead and 100% dead. CVA using principal components also differentiated between the spectra of the differentially treated cells at a 95% confidence level, and Cooman plots showed clear separation between clusters of spectra of bacteria exposed to the different inactivation treatments. Mahalanobis distances (MD) corroborated the results of CVA. Conclusions:  These results demonstrated the effectiveness of rapid cell collection and FT‐IR spectroscopy techniques to differentiate between live and dead E. coli O157:H7 cells. Significance and Impact of the Study:  This technique has potential applications for use with foods subjected to various inactivation treatments.
Subject(s)bacteria , biochemistry , biology , chemistry , enterobacteriaceae , escherichia coli , gene , genetics , microbiology and biotechnology , physics , quantum mechanics , spectroscopy
Language(s)English
SCImago Journal Rank0.889
H-Index156
eISSN1365-2672
pISSN1364-5072
DOI10.1111/j.1365-2672.2011.05215.x

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