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Use of ceramic‐based cell immobilization to produce 1,3‐propanediol from biodiesel‐derived waste glycerol with Klebsiella pneumoniae
Author(s) -
Gungormusler M.,
Gonen C.,
Azbar N.
Publication year - 2011
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2011.05137.x
Subject(s) - 1,3 propanediol , bioreactor , glycerol , biodiesel production , biodiesel , fermentation , hydraulic retention time , raw material , food science , pulp and paper industry , chemistry , wastewater , biochemistry , environmental science , environmental engineering , catalysis , organic chemistry , engineering
Aims: The feasibility of the continuous production of a valuable bioplastic raw material, namely 1,3‐propanediol (1,3‐PDO) from biodiesel by‐product glycerol, using immobilized cells was investigated. In addition, the effect of hydraulic retention time (HRT) was also analysed. Methods and Results: Ceramic balls and ceramic rings were used for the immobilization of a locally isolated strain; Klebsiella pneumoniae (GenBank no. 27F HM063413 ). HRT of 1 h is the best one in terms of volumetric production rate (g 1,3‐PDO l −1 h −1 ). The results indicated that ceramic‐based cell immobilization achieved a 2‐fold higher production rate (10 g 1,3‐PDO l −1 h −1 ) in comparison with suspended cell system (4·9 g 1,3‐PDO l −1 h −1 ). Conclusions: Continuous cultures with immobilized cells revealed that 1,3‐PDO production was more effective and more stable than suspended culture systems. Furthermore, cell immobilization had also obvious benefits especially for resistance of the production for extreme conditions (high organic loading rates, cell washouts). The results were important for understanding the significance of continuous immobilization process among other well‐known 1,3‐PDO fermentation processes. Significance and Impact of the Study: This work is a promising process for further studies, as the immobilized micro‐organism was able to reach high volumetric production rates at short HRT, it has an important role in tolerating and converting glycerol during fermentation. Therefore, HRT is a very significant operational parameter ( P value <0·05) directly affecting the bioreactor performance and production rate.