Premium
Typing of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS 621 multiplex PCR‐based fingerprinting
Author(s) -
Mainil J.G.,
Bardiau M.,
Ooka T.,
Ogura Y.,
Murase K.,
Etoh Y.,
Ichihara S.,
Horikawa K.,
Buvens G.,
Piérard D.,
Itoh T.,
Hayashi T.
Publication year - 2011
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2011.05089.x
Subject(s) - biology , multiplex polymerase chain reaction , microbiology and biotechnology , typing , multiplex , polymerase chain reaction , virology , genetics , gene
Aims: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS 621 . Methods and Results: Two multiplex PCRs, targeting either the left (5′) or right (3′) IS/chromosome junction of 12 IS 621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty‐eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy‐one per cent of the 45 EHEC and EPEC with identical IS 621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had <85% similarity between each others. Epidemiologically related EHEC from each of three independent outbreaks in Japan and Belgium also exhibited identical IS 621 fingerprints and PFGE profiles. Conclusions: The IS 621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory. Significance and Impact of the Study: The IS 621 printing method represents a rapid (24 h) first‐line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks.