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The development of a liquid formulation of Pseudoxanthomonas sp. RN402 and its application in the treatment of pyrene‐contaminated soil
Author(s) -
Nopcharoenkul W.,
Pinphanichakarn P.,
Pinyakong O.
Publication year - 2011
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2011.05037.x
Subject(s) - pyrene , bioaugmentation , biodegradation , contamination , chemistry , glycerol , chromatography , microcosm , bacteria , environmental chemistry , degradation (telecommunications) , pollutant , microorganism , microbiology and biotechnology , food science , organic chemistry , biology , ecology , telecommunications , computer science , genetics
Aim: To develop a liquid formulation of Pseudoxanthomonas sp. RN402 for prolonged storage and maintaining high survival rates and pyrene biodegradability. Methods and Results: Liquid formulations of RN402, designated as L‐RN402, were prepared by suspending bacterial cells (10 9 CFU ml −1 ) in various buffers. Analysis found that phosphate buffer containing glycerol maintained high survival rate (94%) as well as pyrene biodegradability of bacteria after a 30‐day storage. This L‐RN402 could be stored at 30°C for at least 6 months. Bioaugmentation treatment with stored L‐RN402 resulted in the complete degradation of pyrene (300 mg kg −1 ) in soil microcosms within 4 weeks. RN402 could be detected by denaturing gradient gel electrophoresis throughout the period; moreover, real‐time PCR indicated the presence of high number of nidA ‐containing bacteria. Conclusions: A liquid formulation of RN402, an effective pyrene degrader, was developed by suspending RN402 in phosphate buffer containing 1% glycerol. This formulation could be stored at 30°C for at least 6 months and maintain high efficacy in the treatment of pyrene‐contaminated soil. Significance and Impact of the Study: This work is the first description of a liquid formulation of pyrene‐degrading bacteria for prolonged storage that retains biological activity for the treatment of environmental pollutants.