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Experimental uptake and retention of pathogenic and nonpathogenic Vibrio parahaemolyticus in two species of clams: Ruditapes decussatus and Ruditapes philippinarum
Author(s) -
LopezJoven C.,
de Blas I.,
RuizZarzuela I.,
Furones M.D.,
Roque A.
Publication year - 2011
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2011.05024.x
Subject(s) - ruditapes , vibrio parahaemolyticus , biology , microbiology and biotechnology , vibrionaceae , shellfish , vibrio , bivalvia , mollusca , aquatic animal , ecology , bacteria , fishery , fish <actinopterygii> , genetics
Aims: To describe uptake and retention of pathogenic and nonpathogenic Vibrio parahaemolyticus in Ruditapes decussatus and Ruditapes philippinarum. Methods and Results: Ruditapes decussatus accumulated greater concentrations of pathogenic and nonpathogenic V. parahaemolyticus than R. philippinarum. These concentrations decreased earlier in R. decussatus . Nonpathogenic V. parahaemolyticus reached higher concentrations (approx. 1 log CFU g −1 in tissues of R. decussatus and more than 1 log CFU g −1 in R. philippinarum ) than pathogenic V. parahaemolyticus at similar times. It also persisted longer in both species of clams (72 h in R. decussatus and 96 h in R. philippinarum ), while pathogenic V. parahaemolyticus persisted 48 h in R. decussatus and 72 h in R. philippinarum . Conclusions: Ruditapes decussatus incorporated both isolates of V. parahaemolyticus faster than R. philippinarum and it eliminated both isolates earlier than R. philippinarum . Under same conditions, nonpathogenic V. parahaemolyticus might survive better than pathogenic V. parahaemolyticus within both species of clam. Significance and Impact of the Study: Pathogenic V. parahaemolyticus is an important cause of foodborne illnesses. This study shows it may be possible to use nonpathogenic V . parahaemolyticus to perform experimentation to evaluate bacterial evolution in clams, developing an in vivo model useful for risk analysis.