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Molecular characterization of erm (B)‐ and mef (E)‐mediated erythromycin‐resistant Streptococcus pneumoniae in China and complete DNA sequence of Tn 2010
Author(s) -
Li Y.,
Tomita H.,
Lv Y.,
Liu J.,
Xue F.,
Zheng B.,
Ike Y.
Publication year - 2011
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2010.04875.x
Subject(s) - streptococcus pneumoniae , microbiology and biotechnology , erythromycin , sequence (biology) , biology , dna sequencing , dna , sequence analysis , genetics , antibiotics
Aims: To characterize the erm (B)‐ and mef (E)‐mediated erythromycin‐resistant Streptococcus pneumoniae clinical isolates obtained from ten hospitals located different cities in China. Methods and Results: Totally 83 S. pneumoniae were collected, and eighteen representative strains of 66 strains that exhibited erythromycin resistance were used for further characterization by antibiograms, serotyping, PFGE, MLST, DNA sequencing of the macrolide‐resistance elements and mapping of the elements on the chromosome. Twelve isolates showed a high‐level resistance to erythromycin, and six other isolates showed a low‐level resistance to erythromycin. Thirteen isolates harboured a Tn 2010 transposon (26·4 kbp) encoding the erm (B), tet (M) and mef (E) genes and were classified into three types by Tn 2010 structures. The remaining five isolates harboured a Tn 6002 transposon (20·9 kbp) encoding the erm (B) and tet (M) genes and were classified into three types by Tn 6002 locations on the chromosome. Three of the Tn 6002 elements were located within the Tn 5252 ‐like element, implying that these composed a large mobile element. The MLST analyses showed that several clones had been disseminated and that the CC271 strains carrying the Tn 2010 element expressing the high‐level resistance to erythromycin were predominant in China. Four new MLST strains, which were designated as ST3262, ST3263, ST3397 and ST3398 were also identified. Conclusions: The erythromycin resistance determinant of S. pneumoniae that had been isolated in China was located in Tn 2010 or the Tn 6002 element and several clones had been disseminated, and the CC271 strains carrying the Tn 2010 element expressing the high‐level resistance to erythromycin were predominant in China. Significance and Impact of the Study: This is the first molecular analysis of erythromycin‐resistant Streptococcus pneumoniae clinical isolates in China, and the first report of the complete nucleotide sequence of Tn 2010 (26 390 bp) .