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F‐RISA fungal clones as potential bioindicators of organic and metal contamination in soil
Author(s) -
Hong J.W.,
Fomina M.,
Gadd G.M.
Publication year - 2010
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2009.04665.x
Subject(s) - bioindicator , environmental chemistry , ribosomal intergenic spacer analysis , microcosm , pollutant , pyrene , contamination , population , soil contamination , chemistry , biology , ecology , ribosomal rna , organic chemistry , biochemistry , internal transcribed spacer , demography , sociology , gene
Aims: This work has examined the effects of a polycyclic aromatic hydrocarbon and selected toxic metals on fungal populations in a soil microcosm. Methods and Results: By using fungal ribosomal intergenic spacer analysis (F‐RISA) in combination with real‐time PCR quantification, four fungi (D63P2‐1, D63C2‐1, D21Cu1‐1 and D63Pb2‐2) with specific primer pairs to each were successfully evaluated for their potential as bioindicators in response to pyrene, copper (Cu) and lead (Pb), supplied singly and in combination. Conclusions: F‐RISA coupled with real‐time PCR is a useful approach for the identification of microorganisms with potential as bioindicators of organic and toxic metal contamination. Significance and Impact of the Study: These bioindicators could be monitored for their population changes that may indicate pollutant‐induced perturbations in a given system.