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Proteomic analysis of outer membrane proteins of Edwardsiella tarda
Author(s) -
Kumar G.,
Sharma P.,
Rathore G.,
Bisht D.,
Sengupta U.
Publication year - 2010
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2009.04627.x
Subject(s) - proteome , membrane protein , biology , bacterial outer membrane , groel , edwardsiella tarda , gel electrophoresis , polyacrylamide gel electrophoresis , microbiology and biotechnology , two dimensional gel electrophoresis , porin , biochemistry , proteomics , enzyme , escherichia coli , gene , membrane
Aims: The purpose of this study was to identify outer membrane proteins (OMPs) of Edwardsiella tarda . Methods and Results: The OMPs from a virulent strain of E. tarda (ET‐7) was extracted using lauroyl sarcosine method. The OMPs were analysed by two‐dimensional polyacrylamide gel electrophoresis (2D‐PAGE), and protein spots were identified using matrix assisted laser desorption/ionization‐time‐of‐flight mass spectrometry. A total of 21 proteins were identified from 24 protein spots observed on the 2D‐PAGE gel. These proteins were identified as GroEL, antigenic proteins, ABC transporters, elongation factors, OmpA, PTSINtr with GAF domain, catalase C, glycolytic enzymes, DnaJ, transcriptional regulator, proteins mraZ and ccdA. Subcellular localizations, β‐barrel OMPs and lipoproteins of identified proteins were predicted using PSORTb, PRED‐TMBB and L ipo P 1.0 programme. Conclusions: Identification, localization and possible functions of OMPs of E. tarda were studied. Significance and Impact of the Study: These proteins could be used for development of novel drug targets, diagnostics or vaccine against edwardsiellosis.