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Probiotic potential of Staphylococcus hominis MBBL 2–9 as anti‐ Staphylococcus aureus agent isolated from the vaginal microbiota of a healthy woman
Author(s) -
Sung C.,
Kim B.G.,
Kim S.,
Joo H.S.,
Kim P.I.
Publication year - 2010
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2009.04485.x
Subject(s) - staphylococcus aureus , microbiology and biotechnology , bacteriocin , probiotic , antimicrobial , biology , staphylococcus , bacteria , antibiotics , genetics
Aims: To isolate and characterize an antagonist for use as probiotic agent in the biocontrol of Staphylococcus aureus . Methods and Results: Bacteria that exhibited antimicrobial activity against Gram‐positive bacteria including Staph . aureus were isolated from 12 healthy women, with Staphylococcus hominis MBBL 2–9 showing the strongest activity. The bacteriocin produced by Staph . hominis MBBL 2–9 was purified by 60% ammonium sulfate saturation, ultrafiltration, HLB cartridge and reverse‐phase HPLC. The molecular weight was estimated as 2038·2 Da by MALDI–TOF mass spectrometry. The antagonist survived up to 2 h in artificial gastric juice (pH 2·5) and grew in the presence of 1% porcine bile extract. In addition, Staph . hominis MBBL 2–9 adhered effectively to HT‐29 epithelial cell line. Conclusion: Staphylococcus hominis MBBL 2–9 exhibited desirable probiotic traits such as acid tolerance, bile resistance and adherence to epithelial cell line. The bacterium also produced a bacteriocin with unique molecular weight and high antimicrobial activity similar to traditional antibiotics. Significance and Impact of the Study: This study is the first report of a bacteriocin‐producing Staph . hominis MBBL 2–9 that has potential for use as a probiotic agent against Staph . aureus .