Premium
Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma
Author(s) -
Wang L.Y.,
Huang Z.L.,
Li G.,
Zhao H.X.,
Xing X.H.,
Sun W.T.,
Li H.P.,
Gou Z.X.,
Bao C.Y.
Publication year - 2010
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2009.04483.x
Subject(s) - beijing , china , chinese academy of sciences , library science , engineering , engineering physics , computer science , geography , archaeology
Aims: Avermectins are major antiparasitic agents used commercially in animal health, agriculture and human infections. To improve the fermentation efficiency of avermectins, for the first time a plasma jet generated by a novel atmospheric pressure glow discharge (APGD) was employed to generate mutations in Streptomyces avermitilis . Methods and Results: The APGD plasma jet, driven by a radio frequency (RF) power supply with water‐cooled and bare‐metallic electrodes, was used as a new mutation method to treat the spores of S. avermitilis . The plasma jet yielded high total (over 30%) and positive (about 21%) mutation rates on S. avermitilis , and a mutated strain, designated as G1‐1 with high productivity of avermectin B1a and genetic stability, was obtained. Conclusions: Because of the low jet temperature, the high concentrations of the chemically reactive species and the flexibility of its operation, the RF APGD plasma jet has a strong mutagenic effect on S. avermitilis . Significance and Impact of the Study: This is a proof‐of‐concept study for the use of an RF APGD plasma jet for inducing mutations in microbes. We have shown that the RF APGD plasma jet could be developed as a promising and convenient mutation tool for the fermentation industry and for use in biotechnology research.