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Combination of 10% EDTA, Photosan, and a blue light hand‐held photopolymerizer to inactivate leading oral bacteria in dentistry in vitro
Author(s) -
Maisch T.,
Wagner J.,
Papastamou V.,
Nerl H.J.,
Hiller K.A.,
Szeimies R.M.,
Schmalz G.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2009.04342.x
Subject(s) - periodontology , dentistry , medicine , library science , computer science
Aims: The goal of this study was to investigate the phototoxicity of Photosan in combination with EDTA and a hand‐held photopolymerizer used in dentistry for light‐curing resins against leading key pathogens in caries, endodontic treatment failures, and periodontitis respectively. Methods and Results: Cellular uptake of Photosan was detected by fluorescence spectroscopy for Streptococcus mutans and Enterococcus faecalis but not for Aggregatibacter actinomycetemcomitans . Addition of 10% EDTA enabled the uptake of Photosan by A. actinomycetemcomitans . Killing of S. mutans and E. faecalis mediated by Photosan and blue light was concentration and light dose dependent, achieving a ≥99·9% (≥3 log 10 reduction) efficacy of bacteria killing. In the presence of 10% EDTA, Photosan induced a reduction of ≥4 log 10 in the viability of A. actinomycetemcomitans at a concentration of 50 μg ml −1 , upon activation at a dose of 9·65 J cm −2 for 60 s. EDTA alone, light alone, and Photosan alone were not able to kill bacteria. Conclusions: Ten per cent EDTA and Photosan cause a potent phototoxicity against oral bacteria upon illumination with a photopolymerizer. Significance and Impact of the Study: Increasing antibiotic resistance and insufficient drug concentrations within the sulcus fluid are responsible for lacking antimicrobial efficacy. This study provides useful information that combination of Photosan, EDTA, and a photopolymerizer may be a potentially powerful tool for the efficient destroying of key oral bacteria.