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The assessment of the antibacterial and antifungal activities of aspirin, EDTA and aspirin–EDTA combination and their effectiveness as antibiofilm agents
Author(s) -
AlBakri A.G.,
Othman G.,
Bustanji Y.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2009.04205.x
Subject(s) - biofilm , antimicrobial , microbiology and biotechnology , candida albicans , aspirin , pseudomonas aeruginosa , broth microdilution , minimum inhibitory concentration , corpus albicans , chemistry , biology , bacteria , biochemistry , genetics
Aims: To evaluate the antimicrobial activities of aspirin, EDTA and an aspirin‐EDTA (A‐EDTA) combination against Pseudomonas aeruginosa , Escherichia coli and Candida albicans in planktonic and biofilm cultures. Methods and Results: Minimal inhibitory concentrations (MIC) and minimal biocidal concentrations (MBC) were determined using twofold broth microdilution and viable counting methods, respectively. Aspirin’s recorded MIC values ranged from 1·2 to 2·7 mg ml −1 . Checkerboard assay demonstrated a synergism in antimicrobial activity upon combination. Aspirin’s minimal biofilm eradication concentration values (MBEC) against the established biofilms ranged between 1·35 and 3·83 mg ml −1 . A complete eradication of bacterial biofilms was achieved after a 4‐h treatment with the A‐EDTA combination. Conclusion: Both aspirin and EDTA possess broad‐spectrum antimicrobial activity for both planktonic and biofilm cultures. Aspirin used at the MBEC for 24 h was successful in eradicating P. aeruginosa , E. coli and C. albicans biofilms established on abiotic surfaces. Moreover, the exposure to the A‐EDTA combination (4 h) effected complete bacterial biofilm eradication. Significance and Impact of the Study: There is a continuous need for the discovery of new antimicrobial agents. Aspirin and EDTA are ‘nonantibiotic drugs’, the combination of which can be used successfully to treat and eradicate biofilms established on abiotic surfaces.