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Characterization of a methane‐utilizing strain and its application for monitoring methane
Author(s) -
Zhao C.G.,
Zheng J.,
Li H.P.,
Wen G.M.,
He Y.Y.,
Yang S.P.,
Dong C.,
Choi M.M.F.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2009.04169.x
Subject(s) - methane , strain (injury) , detection limit , 16s ribosomal rna , biosensor , klebsiella , gas chromatography , klebsiella pneumoniae , bacterial strain , chemistry , environmental chemistry , bacteria , microbiology and biotechnology , chromatography , biology , escherichia coli , gene , biochemistry , organic chemistry , genetics , anatomy
Aims: To explore new resources of methane‐utilizing micro‐organism and develop a microbial biosensing system for monitoring methane released from natural and semi‐natural ecosystems. Methods and Results: A methane (CH 4 )‐utilizing bacterial strain was isolated from paddy soil using CH 4 as the sole carbon source and identified as Klebsiella sp. ME17 by phenotyping and 16S rDNA sequence analysis. The efficiency of CH 4 utilization of strain ME17 was 83·2% by gas chromatography analysis. A microbial biosensing system for CH 4 detection was developed by combining immobilized cells of strain ME17 with a dissolved oxygen sensor. It was found that response time of the system to CH 4 was <90s. The dissolved O 2 consumption increased with increasing CH 4 from 0% to 16·0% (v/v) demonstrating a positive linear relationship with a low detection limit of 0·2% (v/v). The relative standard deviation is 3·48%. Conclusions: Klebsiella sp. ME17 isolate is capable of utilizing CH 4 . The microbial biosensing system of strain ME17 has been successfully applied to measure standard CH 4 sample with satisfactory results. Significance and Impact of the Study: This study suggests that certain strains of Klebsiella genus are capable of utilizing CH 4 . Our proposed method appears very attractive for CH 4 measurement in coal mine.