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Selective production of cis ‐9, trans ‐11 isomer of conjugated linoleic acid from trans‐ vaccenic acid methyl ester by Delacroixia coronata
Author(s) -
Ando A.,
Ogawa J.,
Sugimoto S.,
Kishino S.,
Sakuradani E.,
Yokozeki K.,
Shimizu S.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.04139.x
Subject(s) - conjugated linoleic acid , linoleic acid , vaccenic acid , conjugated system , chemistry , cis–trans isomerism , biochemistry , biology , stereochemistry , organic chemistry , fatty acid , polymer
Aims: Bio‐process development for isomer selective and efficient production of cis ‐9, trans ‐11‐octadecadienoic acid (CLA) from trans ‐vaccenic acid ( t ‐VA, trans ‐11‐octadecenoic acid) through microbial fatty acid Δ9‐desaturation reaction. Methods and Results: A total of 550 strains of fungi and yeasts were screened for CLA production from t ‐VA through Δ9 desaturation. Delacroixia coronata IFO 8586 was selected as a potent producer of CLA from t ‐VA. Efficient CLA production was observed during cultivation in medium supplemented with the methyl ester of t ‐VA ( t ‐VAME). Under the optimal conditions with 33·3 mg ml −1 of t ‐VAME as substrate, 10·5 mg ml −1 CLA was produced by D. coronata IFO 8586 after 7 days of cultivation in the medium containing dextrin (5·0%), tryptone (2·0%) and thiourea (0·83 μmol ml −1 ). The strain produced the cis ‐9, trans ‐11 isomer of CLA selectively (98% of total CLA), with a small amount of the trans ‐9, trans ‐11 isomer (2% of total CLA), mainly in the form of triacylglycerols (69% of total CLA). Conclusions: A practical bio‐process for selective production of cis ‐9, trans ‐11 isomer of CLA using filamentous fungus D. coronata IFO 8586 was successfully established. Significance and Impact of the Study: Isomer selective bio‐process for the practical production of cis ‐9, trans ‐11‐CLA was first established. The process is benefitable for expanding the application of CLA for medicinal and nutraceutical purposes.