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Optimization of a reconstituted skim milk based medium for enhanced CLA production by bifidobacteria
Author(s) -
Hennessy A.A.,
Ross R.P.,
Devery R.,
Stanton C.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.04098.x
Subject(s) - skimmed milk , food science , actinomycetaceae , milk production , biology , chemistry , bifidobacterium , microbiology and biotechnology , lactobacillus , fermentation , zoology
Aims: To determine the effect of a range of supplements on the bioconversion of linoleic acid to conjugated linoleic acid (CLA) by Bifidobacterium breve NCIMB 702258 in reconstituted skim milk (RSM). Results: Seven supplements (yeast extract, casein hydrolysate, tryptone, l ‐cysteine hydrochloride, sodium acetate, sodium butyrate and sodium propionate) were identified as increasing the bioconversion of linoleic acid to c9 , t 11 CLA. Using these supplements, the percentage bioconversion of linoleic acid (0·35 mg ml −l ) to the c9 , t 11 CLA isomer was elevated from 15·5 ± 1·1% in 20% RSM (w/v) to 48·1 ± 2·2% in the supplemented RSM. Through additional supplementation of 20 mg m1 −1 inulin and optimization of inoculum and linoleic acid concentration, the percentage bioconversion to c9 , t 11 CLA was increased to 55·0 + 3·2%. Conclusions: Through supplementation, the concentration of CLA produced by bifidobacteria in RSM can be increased to levels comparable to those observed in the synthetic medium cys‐MRS. Significance and Impact of the Study: The impact of 22 supplements on the production of the c9 , t 11 CLA isomer by the strain B. breve NCIMB 702258 in milk has been determined. The results provide an understanding of the factors, which influence CLA production by bifidobacteria in RSM.