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The improvement of amorpha‐4,11‐diene production by a yeast‐conform variant
Author(s) -
JiangQiang K.,
Wei W.,
LiNa W.,
XiaoDong Z.,
KeDi C.,
Ping Z.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.04063.x
Subject(s) - yeast , saccharomyces cerevisiae , transformation (genetics) , biology , gene , heterologous expression , heterologous , biochemistry , recombinant dna
Aims: To investigate the effect of the yeast‐conform variant of the Artemisia annua gene encoding for amorpha‐4,11‐diene synthase (ADS) on the production of amorpha‐4,11‐diene in a transformed yeast. Methods and Results: The ADS gene was mutated to the yeast‐conform variant ADSm . The ADSm synthesis was performed based on step‐by‐step extension of a short region of the gene through a series of polymerase chain reactions (PCR). The artificial ADSm gene contained codons preferred by the yeast translation machinery. The sequence was then integrated into a yeast expression vector pYeDP60. The fusion construct was active and the transformed yeast cells produced higher level of amorpha‐4,11‐diene compared with the plant gene‐transformed yeast cells. Conclusions: Strains transformed with the yeast‐conform allele ( ADSm ) were more efficient in terms of production of amorpha‐4,11‐diene than those transformed with the plant gene. Significance and Impact of the Study: We demonstrated that yeast‐conform allele of foreign genes by serial PCR reactions can be a solution to low efficiency of heterologous gene expression in Saccharomyces cerevisiae cells.