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Enhanced biofilm formation and 3‐chlorobenzoate degrading activity by the bacterial consortium of Burkholderia sp. NK8 and Pseudomonas aeruginosa PAO1
Author(s) -
Yoshida S.,
Ogawa N.,
Fujii T.,
Tsushima S.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.04027.x
Subject(s) - biofilm , burkholderia , microbiology and biotechnology , pseudomonas aeruginosa , bacteria , strain (injury) , pseudomonas , pseudomonadaceae , biology , pseudomonadales , biodegradation , chemistry , ecology , genetics , anatomy
Aims: To characterize biofilm formation of a chlorobenzoates (CBs) degrading bacterium, Burkholderia sp. NK8, with another bacterial species, and the biodegradation activity against CBs in the mixed‐species biofilm. Methods and Results: Burkholderia sp. NK8 was solely or co‐cultured with each of five other representative bacteria in microtitre dishes. Biofilm formation involving the strain NK8 was synergistically promoted by co‐culturing with only Pseudomonas aeruginosa PAO1. Epifluorescent microscopy revealed that cells of the bacterial strain NK8 were viable and distributed randomly in the mixed‐species biofilms. Enumeration of the attached cells on the surface of wells revealed that cells of the strain NK8 increased approx. 10‐fold by the co‐culture with the strain PAO1 compared to those by monoculture of the strain NK8, and the degradation activity of 3‐chlorobenzoate by the dual‐species biofilms was more promoted than that by the strain NK8‐monocultured biofilms. Conclusions: Enhanced biofilm formation of Burkholderia sp. NK8 by the bacterial consortium occurred, but is determined by the partner bacterial species. The mixed‐species biofilms have the advantage to degrade CBs on a solid surface. Significance and Impact of the Study: This study provides a significance of bacterial consortia on the biofilm formation and the degradation activity of Burkholderia sp. NK8, which contribute for complete degradation of chlorinated aromatics.