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Methylobacterium sp. resides in unculturable state in potato tissues in vitro and becomes culturable after induction by Pseudomonas fluorescens IMGB163
Author(s) -
Podolich O.,
Laschevskyy V.,
Ovcharenko L.,
Kozyrovska N.,
Pirttilä A.M.
Publication year - 2009
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.03951.x
Subject(s) - axenic , methylobacterium , biology , pseudomonas fluorescens , bacteria , microbiology and biotechnology , inoculation , methylotroph , pseudomonas , 16s ribosomal rna , botany , horticulture , genetics
Aims: To induce growth of endophytic bacteria residing in an unculturable state in tissues of in vitro ‐grown potato plantlets. To isolate and identify the induced bacteria and to localize the strains in tissues of in vitro ‐grown potato plantlets. Methods and Results: The inoculation of in vitro ‐grown potato plants with Pseudomonas fluorescens IMBG163 led to induction of another bacterium, a pink‐pigmented facultative methylotroph that was identified as Methylobacterium sp . using phylogenetic 16S rDNA approach . Two molecular methods were used for localizing methylobacteria in potato plantlets: PCR and in situ hybridization (ISH/FISH). A PCR product specific for the Methylobacterium genus was found in DNA isolated from the surface‐sterilized plantlet leaves. Presence of Methylobacterium rRNA was detected by ISH/FISH in leaves and stems of inoculated as well as axenic potato plantlets although the bacterium cannot be isolated from the axenic plants. Conclusion: Methylobacterium sp. resides in unculturable state within tissues of in vitro ‐grown potato plants and becomes culturable after inoculation with P. fluorescens IMBG163. Significance and Impact of the Study: In order to develop endophytic biofertilizers and biocontrol agents, a detailed knowledge of the life‐style of endophytes is essential. To our knowledge, this is the first report on increase of the culturability of endophytes in response to inoculation by nonpathogenic bacteria.