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Spatio‐temporal analysis of infra‐specific genetic variations among a Pseudomonas aeruginosa water network hospital population: invasion and selection of clonal complexes
Author(s) -
Lavenir R.,
Sanroma M.,
Gibert S.,
Crouzet O.,
Laurent F.,
Kravtsoff J.,
Mazoyer M.A.,
Cournoyer B.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.03907.x
Subject(s) - biology , pseudomonas aeruginosa , microbiology and biotechnology , population , genetic diversity , virulence , tap water , biofilm , clone (java method) , genetics , gene , bacteria , medicine , environmental health , environmental engineering , engineering
Aims: To investigate infra‐specific spatio‐temporal dynamics of a hospital water network Pseudomonas aeruginosa population. To infer the origin of water network isolates and assess their potential health hazard. Methods and Results: 168 P. aeruginosa strains were isolated from tap waters and swabs of tap nozzle aerators of a hospital unit, over 2 years, and from rectal swabs and nosocomial infections. Genetic diversity among this collection was assessed by pulsed field gel electrophoresis of Spe I restricted genomic DNA. Virulence gene sets, biofilm properties, and hypochlorite resistance were analysed. Exactly 68% of the water samples and 74% of the tap nozzle aerators harboured P. aeruginosa . The strains were divided into 22 clonal lineages, with one dominant clone shown to have been involved in a nosocomial infection. Conclusions: An important turnover among the P. aeruginosa hospital population was observed. Some clonal lineages were found to persist, spread in the unit, and diversify into clonal complexes. Rectal carriage appeared an important source of contamination of the water network. Significance and Impact of the Study: High P. aeruginosa infra‐specific population diversity suggested a broad ability in colonizing water networks but persistence analysis indicated a strong selection leading to the emergence of dominant clones.