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Differential expression of plg genes from Penicillium griseoroseum : plg 1 a pectinolytic gene is expressed in sucrose and yeast extract
Author(s) -
Bazzolli D.M.S.,
Ribon A. de O.B.,
Reis K.C.P.,
De Queiroz M.V.,
De Araújo E.F.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.03905.x
Subject(s) - library science , biology , microbiology and biotechnology , computer science
Aims: To study the regulation of the plg 1 and plg 2 genes of Penicillium griseoroseum , in order to identify the industrial potential of their products in alternative carbon sources that are cheaper and widely available in Brazil. Methods and Results: RT‐PCR and Northern blot were used to investigate if plg 1 and plg 2 expression is under influence of catabolic repression, ambient pH and cAMP. Results demonstrated that the genes were differentially regulated depending on the carbon sources in the culture medium and pH. Sucrose, a noninducing carbon source of the pectinolytic system, was able to promote plg 1 transcription but only when yeast extract was added into the culture medium. Conclusions: The plg genes are differentially expressed. The plg 1 gene is more attractive for industrial use due to its expression in alternative carbon sources like sucrose and yeast extract. Significance and Impact of the Study: In recent years, industries have been trying to replace the toxic conventional treatments employed in these processes by more eco‐friendly enzyme treatment. Alternative carbon sources will be tested with the aim to reduce the costs associated to pectin lyase production in Brazil.