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Recombinant Bacillus thuringiensis strain shows high insecticidal activity against Plutella xylostella and Leptinotarsa decemlineata without affecting nontarget species in the field
Author(s) -
Wang G.,
Zhang J.,
Song F.,
Gu A.,
Uwais A.,
Shao T.,
Huang D.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.03866.x
Subject(s) - leptinotarsa , bacillus thuringiensis , plutella , biology , strain (injury) , pest analysis , colorado potato beetle , bacillaceae , larva , microbiology and biotechnology , botany , bacteria , genetics , bacillus subtilis , anatomy
Aims: To construct a recombinant Bacillus thuringiensis (Bt) strain with broad insecticidal spectrum and investigate its impact on nontarget organisms in field. Method and Results: The cry ‐type gene of wild Bt strain UV17 was identified and a novel cry1Ba gene was cloned. The cry3Aa7 gene, which was highly toxic to coleopteran pests, was introduced into UV17, and a recombinant strain designated as UV173A was obtained. Bioassay results showed that UV173A was not only highly toxic against Plutella xylostella (50% lethal concentration [LC 50 ] = 18·03 μg ml –1 ), but also against coleopteran Leptinotarsa decernlineata (LC 50 = 0·19 mg ml –1 ). The recombinant strain was then tested in field trials to monitor its spatial variation of population and to investigate the impact on nontarget invertebrates. Conclusions: A recombinant Bt stain UV173A with broad insecticidal spectrum was obtained, and it did not cause adverse effects on the population of nontarget organisms. Significance and Impact of the Study: The results obtained here indicated that cry1Ba3 gene may be useful for the resistance management of P. xylostella , and the recombinant stain UV173A was potential for field application against some crucifer vegetable pests as well as L. decemlineata.