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Towards a one‐step Enterobacter sakazakii enrichment
Author(s) -
Fox E.M.,
Jordan K.N.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.03836.x
Subject(s) - enterobacter , food science , isolation (microbiology) , biology , cronobacter sakazakii , enrichment culture , microbiology and biotechnology , bacteria , infant formula , escherichia coli , biochemistry , genetics , gene
Aims: The current international standard method for detection of Enterobacter sakazakii from milk products is by the International Organization for Standardization and the International Dairy Federation documented method, a procedure involving two‐step enrichment. This study aimed to assess enrichment of E. sakazakii using a one‐step enrichment. Methods and Results: Enrichment of four strains of E. sakazakii was compared using five different media, with stressed or unstressed cells, and at three levels of competing microflora, which were included to assess their effects on the positive isolation of E. sakazakii . Enrichment of milk powders, prepared by spray‐drying milk seeded with E. sakazakii , was assessed using one‐step enrichment for detection of E. sakazakii , followed by confirmation of positive isolates by real‐time PCR. Conclusions: Current media are unsuitable for enrichment and detection of all E. sakazakii isolates, in particular, when high levels of background microflora are present in the sample matrix, and new defined media are needed for successful one‐step enrichment. Significance and Impact of the Study: These findings provide further analysis of one‐step enrichment processes for E. sakazakii in the presence of competing microflora, and show that further formulation is needed for a universal E. sakazakii enrichment medium, with careful selection of both nutrients and selective agents.