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Effect of incubation temperature on growth parameters of Pseudoalteromonas antarctica NF 3 and its production of extracellular polymeric substances †
Author(s) -
Nevot M.,
Deroncelé V.,
Montes Mª J.,
Mercade E.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.03769.x
Subject(s) - gompertz function , pseudoalteromonas , population , extracellular , chemistry , growth rate , arrhenius equation , arrhenius plot , food science , biochemistry , mathematics , activation energy , statistics , organic chemistry , demography , 16s ribosomal rna , geometry , sociology , gene
Aim: To evaluate the effect of temperature on growth parameters and on extracellular polymeric substance (EPS) production for Pseudoalteromonas antarctica NF 3 . Methods and Results: For this purpose, three growth parameters, lag time ( λ ), maximum growth rate ( μ ) and maximum population density ( A ), were calculated with the predictive Gompertz model. To evaluate the variations in μ with respect to temperature, the secondary Arrhenius and the square root models were used. Below the optimal growth temperature (17·5°C), the growth of P. antarctica was separated into two domains at the critical temperature of 12°C. Within the suboptimal domain (12–17·5°C), the temperature characteristic was the lowest (5·29 kcal mol −1 ). Growth population densities were maintained over the entire physiological portion assayed (5–17·5°C). Higher crude EPS production was found at temperatures included in the cold domain (5–12°C). Conclusions: All calculated parameters revealed an optimal adaptation of this strain to cold temperatures. Significance and Impact of the Study: The knowledge of the influence of temperature on growth parameters of P. antarctica NF 3 and on EPS production could improve the production of this extracellular polymeric substance that is currently being used in the cosmetic and pharmaceutical industries.