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Response of Listeria monocytogenes to liquid smoke
Author(s) -
Guilbaud M.,
Chafsey I.,
Pilet M.F.,
Leroi F.,
Prévost H.,
Hébraud M.,
Dousset X.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2008.03731.x
Subject(s) - listeria monocytogenes , microbiology and biotechnology , incubation , chemistry , protease , proteome , phenol , bacteria , biology , food science , enzyme , biochemistry , genetics , organic chemistry
Aims: To investigate the effect of liquid smoke on growth, survival, proteomic pattern and haemolytic potential of Listeria monocytogenes. Methods and Results: Growth and survival curves were recorded in brain–heart infusion broth supplemented with three concentrations of liquid smoke. L. monocytogenes growth was inhibited in the presence of 15 μg ml −1 phenol while a rapid decrease in cell viability occurred in the presence of 30 μg ml −1 phenol. The proteome of L. monocytogenes cytosoluble proteins was slightly modified after 2‐h incubation with 30 μg ml −1 phenol but no protein already characterized in response to other known stresses was induced, except the protease ClpP. Liquid smoke inhibited the haemolytic potential without affecting hly gene expression, showing a potential inhibition of protein activity or stability. Conclusions: The presence of liquid smoke in a rich medium strongly affected growth and survival of L. monocytogenes . Brief smoke stress affected the metabolic pathways and inhibited the haemolytic activity of L. monocytogenes . Significance and Impact of Study: This study is a first step in the investigation of the influence of a smoked product on L. monocytogenes strains.