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Effect of cultivation conditions on spore production from Bacillus amyloliquefaciens B128 and its antagonism to Botrytis elliptica
Author(s) -
Tzeng Y.M.,
Rao Y.K.,
Tsay K.J.,
Wu W.S.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2007.03683.x
Subject(s) - china , bacillus amyloliquefaciens , botrytis , library science , antagonism , horticulture , biology , political science , microbiology and biotechnology , botrytis cinerea , law , biochemistry , receptor , food science , fermentation , computer science
Aims: To maximize spore production by Bacillus amyloliquefaciens B128, and its antagonism to the fungal pathogen Botrytis elliptica B061. Methods and Results: In the 5‐l stirred‐tank bioreactor (STR), with the 0·5 vvm aeration rate, an agitation rate of 200 rev min −1 significantly enhanced the spore yield compared to the same in 300 rev min −1 cultivations. In a 20‐l airlift bioreactor (ALR) the maximal spore production was further increased with a controlled aeration rate of 2·5 vvm operated in a 24‐mesh net‐draft tube mode, and no pH control cultivation. This spore yield in the 20‐l ALR was five‐ and eightfold higher; in addition the cultivation period was 19 h shorter, compared to that obtained from shaker flask and in the 5‐l STR cultivations respectively. Conclusions: Although culture conditions are still to be optimized, by using an ALR with net‐draft tube, a scaling up from shaker flasks and STR to ALR of spore production by the strain B128 is technically feasible. Significance and Impact of the Study: The spore yields obtained using bioreactors were much higher than those previously reported. The freshly produced spore preparations from the B128 strain significantly antagonized the grey mould pathogen B. elliptica .