Premium
Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT‐33 metabolites on colon cancer carcinoma cells
Author(s) -
Jeong S.Y.,
Park S.Y.,
Kim Y.H.,
Kim M.,
Lee S.J.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2007.03615.x
Subject(s) - library science , medicine , computer science
Aims: The objective of this research was to isolate and identify a cytotoxic marine bacterium, BIT‐33, and to investigate the apoptosis effects of its metabolite on colon cancer cells. Method and Results: We isolated 93 marine bacteria from seawater samples. Of these, strain BIT‐33 exhibited the strongest cytotoxic activity on three colon cancer cells (HT‐29, SW480 and HCT116). Biochemical tests and 16S rDNA sequencing of this strain allowed us to identify BIT‐33 as a strain of Bacillus vallismortis . The cytotoxic compound from B. vallismortis BIT‐33 was purified by reverse‐phase high‐performance liquid chromatography. Direct cytotoxic effect of the compound was measured by 3‐[4,5‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazoliumbromide assay. The compound induced apoptosis of colon cancer cells, as indicated by DNA fragmentation of agarose gel electrophoresis, flow cytometric analysis (sub‐G 1 method) and annexin V staining. Conclusion: The cytotoxic compound from B. vallismortis BIT‐33 was purified, and the compound showed direct cytotoxic and apoptotic effects on colon cancer cells in a dose‐ and time‐dependent manner. Significance and Impact of the Study: Taken together, our results suggest that the compound from B. vallismortis BIT‐33 could be a candidate for the development of apoptosis‐specific anti‐tumour agents. This study indicated that marine bacteria could be an important source of cytotoxic metabolites.