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Peptones from diverse sources: pivotal determinants of bacterial growth dynamics
Author(s) -
Gray V.L.,
Müller C.T.,
Watkins I.D.,
Lloyd D.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2007.03577.x
Subject(s) - sterilization (economics) , gelatin , food science , casein , bacterial growth , bacteria , yeast , salmonella , yeast extract , chemistry , growth medium , inoculation , biology , microbiology and biotechnology , biochemistry , fermentation , monetary economics , economics , foreign exchange market , genetics , foreign exchange , immunology
Aims: In view of the major problems encountered by microbiologists in obtaining reproducible data on growth dynamics in complex media, we studied the effects of different peptones made from different biological sources and produced by numerous manufacturers. Methods and Results: Peptones (including casein, gelatin, meat, soy and yeast) were assessed as a constituent of the pre‐enrichment broth buffered peptone water (BPW). Generation times ( g ) and yields of Salmonella serovar Typhimurium were significantly affected by the type of peptone employed with yeast peptones generating yields of 7·04 × 10 9 CFU ml −1 and gelatin peptones producing 0·81 × 10 9 CFU ml −1 . Medium sterilization was also found to have significant effects ( P = 0·000) upon subsequent bacterial growth. Filter sterilization of BPW media produced lower generation times compared with those obtained after sterilization by autoclaving. Finally, it was observed that some peptones which produced good growth when inoculated with healthy organisms, showed relatively poor growth when inocula were sublethally injured by heating. Conclusions: Variation in peptone as a constituent of BPW has a significant effect on growth and enumeration of bacteria. Significance and Impact of the Study: Increased consideration with respect to culture media may significantly improve bacterial growth and experimental reproducibility.