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Genotypic characterization of Enterobacter sakazakii isolates by PFGE, BOX‐PCR and sequencing of the fliC gene
Author(s) -
Proudy I.,
Bouglé D.,
Coton E.,
Coton M.,
Leclercq R.,
Vergnaud M.
Publication year - 2008
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2007.03526.x
Subject(s) - pulsed field gel electrophoresis , biology , genotyping , microbiology and biotechnology , typing , genotype , enterobacter , multilocus sequence typing , flagellin , gene , genetics , escherichia coli
Aims: Enterobacter sakazakii is an emerging food‐borne pathogen that can cause rare but severe forms of neonatal meningitis, bacteraemia and necrotizing enterocolitis. A rapid typing method at the strain level is needed to determine the monoclonality or polyclonality of the isolates during outbreaks. Methods and Results: The BOX‐PCR fingerprinting technique, which targets the repetitive BOX sequences, and sequencing of the flagellin gene, fliC , were evaluated against a panel of 27 Ent. sakazakii strains from clinical and environmental sources. The typeability and discriminatory power of the techniques were compared with those of pulsed‐field gel electrophoresis (PFGE), the reference genotyping method. BOX‐PCR results yielded 92% agreement with PFGE results, whereas fliC gene sequencing was poorly discriminative. Conclusions: In our study, BOX‐PCR and PFGE were similarly discriminatory to type Ent. sakazakii strains. The weak variability of the Ent. sakazakii fliC gene was related to the absence of the variable central domain present in most fliC genes of Enterobacteriaceae. Significance and Impact of the Study: The BOX‐PCR typing provides an accurate discrimination and a rapid answer to identify clonal isolates of Ent. sakazakii .