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Characterization of dehydromonacolin‐MV2 from Monascus purpureus mutant
Author(s) -
Dhale M.A.,
Divakar S.,
UmeshKumar S.,
Vijayalakshmi G.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2007.03457.x
Subject(s) - monascus purpureus , monascus , mutant , biology , chemistry , microbiology and biotechnology , food science , biochemistry , fermentation , gene
Aim: Characterization of dehydromonacolin‐MV2, a bioactive metabolite isolated from Monascus purpureus mutant (CFR 410‐11) . Methods and Results: Chloroform extract of rice, fermented with a hyperpigment‐producing mutant of M. purpureus (CFR 410‐11) was found to contain metabolites that inhibited the growth of Bacillus, Pseudomonas and Streptococcus in agar gel diffusion assays. The extract inhibited lipid peroxidation and scavenged 2,2‐diphenyl‐1‐pycrylhydrazyl and hydroxyl radicals. The active compound purified by silica gel column chromatography was characterized by NMR. The carbon, proton and 2D HSQCT assignments identified dehydromonacolin‐MV2 as the bioactive metabolite. Conclusions: Dehydromonacolin‐MV2 apparently originated in the mutant by hydroxylation and oxidation of monacolin‐J, an intermediate of monacolin biosynthetic pathway. Significance and Impact of the Study: Identification of the production of dehydromonacolin‐MV2 by M. purpureus mutant (CFR 410‐11) is new to literature. Bioactive properties of the compound suggested its pharmaceutical applications.