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Bacillus pumilus SG2 isolated from saline conditions produces and secretes two chitinases
Author(s) -
Ahmadian G.,
Degrassi G.,
Venturi V.,
Zeigler D.R.,
Soudi M.,
Zanguinejad P.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2007.03340.x
Subject(s) - chitinase , bacillus pumilus , biology , bacillaceae , bacillales , biochemistry , chitin , microbiology and biotechnology , halotolerance , bacillus subtilis , gene , bacteria , genetics , chitosan
Aims: Isolation and characterization of chitinases from a halotolerant Bacillus pumilus . Methods and Results: Bacillus pumilus strain SG2 was isolated from saline conditions. It is able to produce chitinase activity at high salt concentration. SDS‐PAGE analysis of the B. pumilus SG2 culture supernatant showed two major bands that were induced by chitin. The amino acid sequence of the two proteins, designated ChiS and ChiL, showed a high homology with the chitinase of B. subtilis CHU26, and chitinase A of B. licheniformis , respectively. N ‐terminal signal peptide of both proteins was also determined. The molecular weight and isoelectric point of the chitinases were determined to be 63 and 74 kDa, and 4·5 and 5·1, for ChiS and ChiL respectively. The genes encoding for both chitinases were isolated and their sequence determined. The regulation of the chitinase genes is under the control of the catabolite repression system. Conclusions: Secreted chitinase genes and their flanking region on the genome of B. pumilus SG2 have been identified and sequenced. Significance and Impact of the Study: This is the first report of a multiple chitinases‐producing B. pumilus halotolerant strain. We have identified two chitinases by using a reverse genetics approach. The chitinases show resistance to salt.