Premium
Avian‐ and mammalian‐derived antibodies against adherence‐associated proteins inhibit host cell colonization by Escherichia coli O157:H7
Author(s) -
Cook S.R.,
Maiti P.K.,
DeVinney R.,
AllenVercoe E.,
Bach S.J.,
McAllister T.A.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2007.03334.x
Subject(s) - intimin , polyclonal antibodies , microbiology and biotechnology , antibody , biology , escherichia coli , antiserum , colonization , virology , virulence , pathogen , enterobacteriaceae , bacteria , immunology , biochemistry , gene , genetics
Aim: To evaluate the potential for polyclonal antibodies targeting enterohaemorrhagic Escherichia coli (EHEC) virulence determinants to prevent colonization of host cells by E. coli O157:H7. Methods and Results: Rats and laying hens were immunized with recombinant proteins from E. coli O157:H7, EspA, C‐terminal intimin or EscF. Rat antisera (IgG) or chicken egg powders (IgY) were assessed for their ability to inhibit growth and colonization‐associated processes of E. coli O157:H7. Mammalian antisera with antibodies to intimin, EspA or EscF effectively reduced adherence of the pathogen to HeLa cells ( P < 0·05) and prevented type III secretion of Tir. Similarly, HeLa cells treated with chicken egg powder containing antibodies against intimin or EspA were protected from EHEC adherence ( P < 0·05). Neither egg nor rat antibody preparations had any antibacterial effect on the growth of EHEC ( P > 0·05). Conclusions: Antibody preparations targeting EHEC adherence‐associated factors were effective at preventing adhesion and intimate colonization‐associated events. Significance and Impact of the Study: This work indicates that immunotherapy with anti‐adherence antibodies can reduce E. coli O157:H7 colonization of host cells. Passive immunization with specific antibodies may have the potential to reduce E. coli O157:H7 colonization in hosts such as cattle or humans.