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Comparative study on effect of different promoters on expression of cry1Ac in Bacillus thuringiensis chromosome
Author(s) -
Chaoyin Y.,
Wei S.,
Sun M.,
Lin L.,
Faju C.,
Zhengquan H.,
Ziniu Y.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.03269.x
Subject(s) - cry1ac , bacillus thuringiensis , biology , promoter , plasmid , gene , chromosome , gene expression , microbiology and biotechnology , genetics , transgene , genetically modified crops , bacteria
Aims: The aim of this work was to investigate the effect of cry3A promoter on the expression of cry1Ac in Bacillus thuringiensis chromosome and stably enhance the production of different cry genes under the control of cry3A promoter. Methods and Results: The cry1Ac gene, which is specific to Lepidopteran larvae, was integrated into the chromosome of a B. thuringiensis plasmid‐free and acrystalliferous strain BMB171, under the control of cry3A promoter and cry1Ac promoter, respectively. The expression of cry1Ac genes in the chromosome of host strain was investigated. The results from sodium dodecyl sulfate–polyacrymide gel electrophoresis, crystal observation and bioassay showed that either integrated with cry3A promoter ( cry3A pro ‐cry1Ac ) or with its native promoter ( cry1Ac pro‐ cry1Ac ), cry1Ac gene could efficiently and stably express in the chromosome. The production of cry3A pro ‐cry1Ac gene was higher than that of cry1Ac pro ‐cry1Ac gene. Conclusions: The cry3A promoter enhanced the expression of cry1Ac gene efficiently either on the chromosome or on the plasmid in B. thuringiensis strain. Significance and Impact of the Study: So far, the comparative studies on cry3A promoter and other cry promoters were carried on B. thuringiensis plasmids. This system offers an additional method for potentially improving the efficacy of B. thuringiensis insecticidal proteins efficiently, stably and safely.