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Cloning, sequencing and characterization of a urease gene operon from urease‐positive thermophilic Campylobacter (UPTC)
Author(s) -
Kakinuma Y.,
Iida H.,
Sekizuka T.,
Usui K.,
Murayama O.,
Takamiya S.,
Millar B.C.,
Moore J.E.,
Matsuda M.
Publication year - 2007
Publication title -
journal of applied microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.889
H-Index - 156
eISSN - 1365-2672
pISSN - 1364-5072
DOI - 10.1111/j.1365-2672.2006.03212.x
Subject(s) - operon , biology , gene , genetics , urease , open reading frame , plasmid , campylobacter jejuni , stop codon , nucleic acid sequence , orfs , helicobacter , structural gene , microbiology and biotechnology , escherichia coli , peptide sequence , biochemistry , bacteria , enzyme , helicobacter pylori
Aims: To clone, sequence and characterize the genetic organization of urease genes within urease‐positive thermophilic Campylobacter (UPTC). Methods and Results: An approx. 5·1‐kbp region encoding a urease gene operon was identified, when recombinant plasmid DNAs from a genomic DNA library of a Japanese isolate (CF89‐12) of UPTC were analysed. Conclusions: Six closely spaced and putative open reading frames (ORFs) for ureA , ureB , ureE , ureF , ureG and ureH were detected. ATG codons initiated each ORF of the UPTC urease operon except for ureB and ureH , which commenced with the most probable TTG codon. Overlaps were detected between ureA and ureB and also between ureB and ureE . Probable ribosome‐binding sites and a putative ρ ‐independent transcriptional termination region were identified. Two putative promoter structures, consisting of consensus sequences at the −35 like and −10 regions were also identified. Significance and Impact of the Study: Construction of a neighbour‐joining tree based on the nucleotide sequence data of urease genes indicated that UPTC formed a cluster with some Helicobacter organisms separate from the other urease‐producing bacteria, suggesting a commonly shared ancestry between UPTC and Helicobacter urease genes.